不同浓度雾化吸入海风藤对急性肺损伤新生大鼠CC16的影响

Effect of Caulis piperis kadsurae on Clara cell protein （CC16） in neonatal rats with acute lung injury

目的探讨不同浓度雾化吸人海风藤对急性肺损伤（Au）新生大鼠Clara细胞蛋白（CC16）的影响。方法150只健康新生SD大鼠注射内毒素（LPS）后随机分为未治疗组（对照组）、海风藤治疗低浓度组（治疗1组）和海风藤治疗高浓度组（治疗2组）。按组别采集肺组织行病理学观察及测肺湿／干质量（W／D）比值，同时收集血清及支气管肺泡灌洗液（BALF），用双抗体夹心ELISA法检测分析CCl6含量。结果①鼠肺外观LPS注射48h后同一时间点，与对照组及治疗1组相比，治疗2组水肿程度轻、出血肺叶面积少。光镜下对照组及治疗1组肺组织切片可见大量红细胞，治疗2组为少量红细胞渗出。②对照组及治疗1、2组肺W／D比值均升高，治疗2组在24h～7d，各亚组与对照组比较差异有统计学意义（P〈0．05）。③各组血清CC16含量均呈先升后降的趋势，治疗2组在24h～7d，各亚组与对照组比较均差异有统计学意义，治疗1组与对照组除72h亚组P〈0．05外，其余差异均无统计学意义（P〉0．05）。各组BALF中CC16水平均迅速下降，48h后逐渐回升，治疗2组在8～72h亚组与对照组比较（P〈0．01），治疗1组在48、72h时间点与对照组比较差异有统计学意义（P〈0．05）。结论雾化吸入高浓度海风藤可减轻Au新生鼠的肺水肿及肺出血程度，海风藤还能减少Clara细胞受损，促进肺微血管对水肿吸收，可能对血管内皮完整性有一定的保护作用。

Objective To discuss the effect of Caulis piperis kadsurae on Clara cell protein （CC16） in neonatal rats with acute lung injury. Methods After injection of lipopolysaccharides （LPS）, 150 healthy neonatal SD rats were randomly divided into untreated group （control group）, Caulis piperis Kadsurae treatment with low concentration group （treatment group 1 ） and treatment with high concentration group （treatment group 2 ）. Each group of rats＇ lung tissue was collected for pathological observation and the lung wet/dry weight ratio was measured, and the serum samples and bronchoalveolar lavage fluid （BALF） was collected, CC16 in serum and BALF was detected using double antibody sandwich ELISA assay. Results ①The rats lung appearance of 48 h after the injection LPS, the treatment group 2 had a lesser degree of pulmonary edema and had small area of pulmonary hemorrhage comparing to control group and treatment group 1. ②Under light microscope, there were a lot of red cells in the control group and the treatment group 1, but treated group 2 had a smaller amount. ③The wet weight / dry weight （W/D） ratio of lung in each group increased, treatment group 2 in 24 h - 7 d groups compared with the control group had significant difference （ P 〈 0.05 ）. ④CC16 in serum were increased first and then decreased, treatment group 2 in 24 h - 7 d groups compared with the control group had statistically significant difference, treatment group 1 and control group except 72 h group （P 〈：0.05 ） , others had no statistically significant difference. Each CC16 in BALF were decreased rapidly at the beginning, then it gradually increased after 48 h, treatment group 2 in 8 - 72 h groups compared with the control group, P 〈0.01, treatment group 1 compared with control group in 48 h, 72 h subgroups P 〈 0.05. Conclusion Atomizing inhalation of high concentrations of Caulis piperis kadsurae can reduce the degree of pulmonary edema and pulmonary hemorrhage of ALl newborn rats, Caulis piperis kadsurae can reduce Clara cell＇s damage, promote absorption of edema, and may have the function of protecting the vascular endothelium.