HPLC法测定浆水中有机酸的含量

Determination of organic acids in Jiangshui by high performance liquid chromatography

建立了高效液相色谱法测定浆水中4种有机酸含量的方法。色谱柱:Agilent ZORBAX SB-AQ柱(250mm×4.6 mm,5μm);流动相:甲醇∶0.01 mol/L KH2PO4溶液(0.5∶99.5,pH=2.0);流速:0.6 mL/min;柱温:30℃;进样量:20μL;DAD检测器;检测波长:210 nm。在优选的色谱条件下,所测的酒石酸、乳酸、乙酸、富马酸得到较好的分离。日内精密度为0.6%～1.8%,日间精密度为1.7%～4.2%,回收率为81.0%～116.0%。

A HPLC method for rapid determination of four organic acids in Jiangshui was developed in this pa- per. Agilent ZORBAX SB-AQ column（250 mm ×4.6 mm, 5μm） was used at 30℃. The mobile phase was CH3OH ：0.01 mol/L KH2PQ（0.5：99.5 ,pH =2.0）. The flow rate was 0.6 mL/min and the detection wavelength was set at 210 nm. The injection volumes were 20 μL for each run. Based on the above optimizing chromatogram conditions, four organic acids, including tartaric acid, lactic acid, acetic acid and fumaric acid, were separated satisfactorily. The intra-day and inter-day precision （ RSD% ） for the analytes ranged from 0.6% to 1.8% and from 1.7% to 4.2% , respectively. The average recovery factors were from 81.0% to 116.0%. The results showed that developed method was successfully applied for the detection and isolation of four organic acids in Jiangshui.