胎次及天数对牦牛初乳活性成分的影响

Influence of parities and the number of days on active ingredients of yak colostrum

采用双抗体一步夹心法酶联免疫吸附实验(ELISA),研究了五个胎次的天祝白牦牛初乳中免疫球蛋白G(IgG)、乳铁蛋白(LF)以及胰岛素样生长因子-I(IGF-I)在产后七天的浓度变化规律,为探讨白牦牛初乳的营养价值及其资源的合理开发和利用提供理论依据。结果表明:天祝白牦牛初乳中IgG、LF和IGF-I在分娩后第1天且四胎浓度最高,分别为2404.41、2001.53和1206.48U/L,其后都随产后泌乳天数的增加急剧下降,5天以后下降速度趋于平缓。产后四天内牛初乳的IgG、LF和IGF-I含量仍可达较高水平,营养价值丰富,具有一定的开发价值,因此在开发利用牦牛初乳产品时,为扩大牛初乳的利用率,可采集四天内初乳进行加工利用。

Double-antibody sandwich enzyme-linked immunosorbent assay （ELISA）was used to detect test samples.This essay mainly discussed the concentration variation of immunoglobulin G （IgG）, lactoferrin （LF）and insulin-like growth factor-Ⅰ（IGF-Ⅰ） in five different parities from Tianzhu white yak＇s colostrum during the lactation of seven days. It will be a theoretical basis to study the nutritional value as well as rational development and utilization of white yak＇ s colostrum.The results showed that the highest concentration point of IgG, LF and IGF-I in yak＇ s colostrums was the birth of the fourth parities on the first day,the numbers respectively reached 2404.41, 200L53and 1206.48U/L.Besides,the number had a sharp decline with the process of lactation and gradually get a moderation four days later.The study showed that the concentration of colostrum IgG, LF and IGF- I can keep higher during the first four days, namely,it had a richful nutrition,which was a good reason for us to research.