陕西苦菜叶总黄酮的提取及抗氧化活性的测定

Optimization for ultrasonic-assisted extraction of total flavonoids from wild patrinia villosa in shaanxi and its antioxidant activity determination in vitro

苦菜总黄酮的提取及抗氧化活性的研究可提高我国野生资源利用和活性成分的开发。以苦菜为原料,采用超声波辅助提取法对苦菜叶总黄酮的提取工艺进行优化,采用分光光度法测定提取物的还原力,对羟基自由基(·OH)、超氧阴离子(O2-·)、DPPH自由基(DPPH·)的清除作用进行研究。结果表明:超声波辅助提取的优化工艺参数为乙醇体积分数80%、提取温度70℃、料液比1∶35、超声时间40min,此条件下总黄酮的提取含量为64.62mg/g。苦菜叶黄酮对羟基自由基、超氧阴离子、DPPH自由基具有良好的清除能力,同时还具有较强的还原能力,活性大小与提取物的浓度呈明显的线性关系。

Extraction of total fiavonoids from wild patrinia villosa and its antioxidant activity determination can make full use of this kind of wild resource and exploit activity component.Single factors experiment and orthogonal array design methods were used to optimize the conditions for ultrasonic-assisted extraction of total flavonoids from wild patrinia villosa The antioxidant activity of the extract was evaluated by spectrophotometry on its ability of scavenging hydroxyl radical（ · OH）, superoxide anion radical （O2- ·） and 1-diphenyl-2-picrylhydrazy（ DPPH · ） ,total reducing power.Results indicated that temperature,solid-liquid ratio, ethanol concentration and extraction time significantly affected the extraction percent of total flavonoids.The optimum procedure for ultrasonic-assisted extraction of total flavonoids were ethanol concentration of 80%, extraction temperature of 70℃, ratio of material to solvent of 1：3.5, ultrasonic time of 40min and that the flavonoids yield was up to 64.62mg/g.The scavenging effects of the extract on · OH,O2- · ,DPPH · were significant,the extract also had a strong total reducing power.All these activities were concentration dependent.