响应面法优化超声波辅助提取石榴籽中原花青素工艺的研究

Study on optimization of the process of ultrasonic assisted extraction of proanthocyanidins in pomegranate seeds by response surface methodology

以安徽怀远石榴的薄皮糙品种为原料,研究石榴籽中原花青素的提取工艺。在单因素实验基础上选取提取功率、pH、液料比和提取溶剂浓度四个实验因素。根据中心组合(Box-Behnken)实验设计原理,采用四因素三水平的响应面分析方法,对各因素的显著性和交互作用进行分析,优化石榴籽中原花青素的提取工艺。结果表明:超声波辅助提取石榴籽中原花青素的最佳工艺条件为功率125W、pH5.0、料液比1∶33、乙醇浓度51%,在此提取条件下,原花青素的得率最高达4.40%,相对误差0.23%。

In this paper,thin-skinned rough of Anhui Huaiyuan pomegranate varieties was selected as raw materials to research proanthocyanidin extraction process in pomegranate seeds.Four factors such as extraction power, pH, solid-liquid ratio and extraction solvent concentration were selected based on single-factor tests. Response surface methodology of four factors and three levels was used to analyze significance and interaction of every factor according to the center combination （ Box- Behnken） experimental design principles, and to optimize the extraction process of procyanidins in pomegranate seeds.The results showed that the optimum conditions for ultrasonic assisted extraction of procyanidins in pomegranate seed was power 125W, pH5.0, solid-liquid ratio 1：33, ethanol concentration of 51% ,and the extraction rate of proanthocyanidins was up to 4.40%, relative error of 0.23% under this extraction conditions.