免疫亲和柱同时净化-HPLC法测定植物油中黄曲霉毒素和玉米赤霉烯酮

Rapid determination of aflatoxin and zearalenone in edible oil by high performance liquid chromatography with immunoaffinity column clean-up

建立了免疫亲和柱同时净化-高效液相色谱法检测植物油中的黄曲霉毒素B1、B2、G1、G2和玉米赤霉烯酮的分析方法。黄曲霉毒素B1、B2、G1、G2的方法的定量限均为0.1μg/kg,玉米赤霉烯酮的方法的定量限为5.0μg/kg,相对标准偏差低于10.4%,回收率为82.0%～95.5%。该方法简便快速、灵敏准确、重现性好,可以用于对植物油中黄曲霉毒素B1、B2、G1、G2和玉米赤霉烯酮进行快速定量检测。

Rapid determination of aflatoxin and zearalenone in edible oil by high performance liquid chromatography with immunoaffinity column clean- up was developed. The limit of quantification of aflatoxin B1, B2, G1, G2 was 0.1μg/kg,the limit of quantification of zearalenone was 5.0μg/kg,the relative standard deviations was below 10.4%. Recoveries of aflatoxin B1, B2, G1, G2 and zearalenone spiked to samples were between 82.0%-95.5%. High performance liquid chromatography with immunoaffinity column clean-up was a rapid, simple and exact method, it could be used for rapid determination of aflatoxin and zearalenone in edible oil.