LC-MS/MS测定马来酸桂哌齐特原料合成中间体残留

Determination of the Synthesized Intermediates Residual of Cinepazide Maleate by LC-MS/MS

目的采用LC-MS/MS测定马来酸桂哌齐特原料中间体氯乙酰吡咯烷、1-哌嗪乙酰基吡咯烷的含量,考察马来酸桂哌齐特原料中两种中间体残留情况。方法建立LC-MS/MS测定中间体氯乙酰吡咯烷、1-哌嗪乙酰基吡咯烷的方法,测定马来酸桂哌齐特原料样品中的中间体杂质残留。结果 LC-MS/MS测定中间体氯乙酰吡咯烷、1-哌嗪乙酰基吡咯方法精密度分别为3.6%和4.0%,线性回归方程分别为Y=26 501X 6 034(r=0.999 8,n=5)及Y=51 616X 10 434(r=0.999 9,n=5),平均回收率分别为98.2%和97.3%,测定马来酸桂哌齐特原料中两中间体均<0.05%。结论该方法准确可靠,灵敏度高,可为马来酸桂哌齐特原料质量控制及质量标准的建立提供依据。

OBJECTIVE To determine the content of chloroacetyl pyrrolidine and 1-piperazine acetyl pyrrolidine which are the intermediates of cinepazide maleate synthesis in cinepazide maleate by LC-MS/MS. METHODS The LC-MS/MS method was established for determination of chloricacetyl pyrrolidine and 1-piperazine acetyl pyrrolidine. The residue of these two intermediates had been determined in cinepazide maleate. RESULTS The precision of the LC-MS/MS for chloroacetyl pyrrolidine and 1-piperazine acetyl pyrrolidine were 3.6% and 4.0%, the linear regression equation were Y=26 501X-6 034 （r=0.999 8, n=5） and Y=51 616X-10 434（r=0.999 9, n=5）, the average recoveries were 98.2% and 97.3%. The contents of two intermediates in cinepazide maleate were both less than 0.05%. CONCLUSION The method is accurate, reliable with high sensitivity, and can provide the basic to quality control and the establishment of quality standard for cinepazide maleate.