麦芽糖假丝酵母降解苯酚的特性及动力学研究

Characteristics and Kinetics of Phenol Degradation by Candida maltosa

从活性污泥中分离得到一株高效降解苯酚菌,经形态特征观察和生理生化试验,鉴定该菌株为麦芽糖假丝酵母。该菌株能以苯酚为唯一碳源和能源,最适宜降解工艺条件如下:温度为30℃,初始pH值为7.0,摇床转速为200 r/min。降解试验表明,该菌能在69 h内完全降解1 800 mg/L苯酚。随着苯酚浓度的增加,底物抑制作用增强。菌株的生长动力学过程符合Hal-dane方程,可利用该方程模拟不同初始浓度苯酚的降解过程。

An effective phenol-degrading strain was isolated from activated sludge, and was identi- fled as Candida maltosa based on morphological, biochemical and physiological experiments. The strain could utilize phenol as sole carbon and energy source. The optimum degradation conditions were tempera- ture of 30℃, initial pH of 7.0 and shaking speed of 200 r/min. The degradation experiment indicated that the strain could completely degrade 1 800 mg/L phenol within 69 h. The higher the phenol concen- tration was, the stronger the substrate inhibition was. Growth kinetics of the strain fitted the Haldane e- quation, which could be used to simulate phenol degradation at different initial concentrations.