大河乌猪血浆胆固醇脂转移蛋白基因多态性与生产性能的相关性分析

Polymorphism of CETP Gene and Association Analysis with Production Performance-related in Dahe Blank Pig

试验应用PCR-RFLP技术对云南培育品种大河乌猪4代(F4)、5代(F5)和6代(F6))共334头个体的血浆胆固醇脂转移蛋白(cholesteryl ester transfer protein,CETP)基因内含子1436bp进行扩增,用限制性内切酶AvaⅡ对其酶切可得到3种基因型:BB、Bb和bb,F4、F5、F6都以b等位基因为优势等位基因,其等位基因频率分别为0.5821、0.6042和0.6885。取不同基因型纯合子样品进行测序,共发现7个单核苷酸多态位点(SNPs),其有3个为转换,4个突变为颠换,多态位点分别位于19、69、132、175、203、224、234bp处。应用最小二乘分析不同基因型和不同性别的基因型对生长性状(初生重、45日龄重、4月龄体重、6月龄体重、体高、体长、管围、背膘厚)的影响。结果显示,F4代Bb和bb基因型个体体长显著高于BB基因型(P<0.05);bb基因型6月龄管围显著高于BB基因型(P<0.05),与Bb基因型个体间差异不显著(P>0.05);F5代Bb和bb基因型个体初生重显著高于BB基因型(P<0.05),两者之间差异不显著(P>0.05);F6代bb基因型个体初生重、45日龄重显著高于BB基因型(P<0.05),与Bb基因型个体间差异不显著(P>0.05);其余性状不同基因型间无显著差异(P>0.05)。另外,bb基因型雄性和雌性个体在初生重都存在显著差异(P<0.05)。研究结果提示,CETP基因对猪生长性状(特别是初生重)存在一定影响;选择带有b等位基因的个体有望提高猪体质量相关的生长性状,该SNP可能是改良猪生长速度性状的重要分子标记位点。

The associations between SNPs in the cholesteryl ester transfer protein （CETP） gene and production performance of pigs were reported in this study, the polymorphism of CETP gene was detected by a AvaⅡ RFLP in 334 Dahe Black pigs across F4 to F6 generations. These results showed that b alleles was excellent gene with frequencies of 0.5821, 0.6042 and 0.6885 in F4, F5 and F6 generations, respectively. Seven SNPs （19, 69, 132, 175, 203, 224 and 234 bp, respectively） were found by the homozygous samples sequencing. Associations between the founded AvaⅡ RFLP and birth weight, body weight at 45 days, body weight at 4 months, body weight at 6 months, body height at 6 months, body length at 6 months and cannon circumference at 6 months and back-fat thickness at 6 months were tested by least square analysis. The results showed that in F4 generation, body length at 6 months with Bb and bb genotype was higher than that with BB genotype （P〈0.05）;cannon circumference at 6 months with bb genotype was higher than that with BB genotype （P〈0.05）, that with Bb genotype was not significant （P〉0.05）. In F5 generation, the birth weight with Bb and bb genotype was higher than that with BB genotype（P〈0.05）, no significant difference between them （P〉0.05）.In F6 generation, the birth weightand body weight at 45 dayswith bb genotype was higher than that with BB genotype （P〈0.05）, that with Bb genotype was not significant （P〉0.05）. In addition,the birth weight showed the higher significant difference between bb genetype and female/male （P〈0.05）. These results suggested that the CETP gene had some effects on the growth traits of porcine,and the selection of b allect was favored with regard to the growth traits of porcine, this SNP might be considered as a potential genetic marker for improving growth.