德宏水牛与尼里拉菲水牛及其杂交水牛的细胞遗传学研究

Cytogenetic Research on Dehong Buffalo,Nili-Ravi Buffalo and Their F1 Hybrid Bulls

为了弄清河川型水牛与沼泽型水牛杂交后代的核型,以及与其育性和亲本核型的关系,用外周血液淋巴细胞培养法对尼里拉菲水牛、德宏水牛及尼里拉菲水牛×德宏水牛F1代公牛进行淋巴细胞培养,制作染色体标本,并进行G-带染色;利用BEION染色体核型分析系统对染色体标本分别进行染色体核型分析,确定其染色体核型。根据染色体核型分析结果,并结合其育性,分析染色体核型与其育性的关系。结果显示,尼里拉菲水牛核型为2n=50,XY;德宏水牛核型为2n=48,-4,-4,-9,-9,+t(4;9),+t(4;9),XY;尼里拉菲水牛×德宏水牛F1代公牛核型为:2n=49,-4,-9,+t(4;9),XY。

In order to make clear the hybrid F1 （Dehong buffalo譔ili-Ravi buffalo） bulls karyotype and fertility and the relationship between them and the parents, the study cultrued lymphocyte in the hybrid F1 （Dehong buffalo, Nili-Ravi buffalo, Nili-Ravi譊ehong buffalo）males by peripheral blood lymphocyte culturing, made chromosomal sample and G-band dyeing. Then chromosome karyotypes in them were analysed and made sure by BEION Karyotyping system. The results showed that the karyotype of Dehong buffalo was 2n=50, XY, Nili-Ravi buffalo was 48,-4,-4,-9,-9,＋t（4;9）,＋t（4;9）,XY and hybrid F1（Nili-Ravi譊ehong buffalo） 49,-4,-9,＋t（4;9）,XY.