中西医结合治疗肾性贫血的红细胞保护机理研究

Mechanism of Protective Effect of High Dosage Erythropoietin(EPO) on Renal Anemia Treated with Combination of Chinese and Western Medicine

目的:评价养血饮联合促红细胞生成素(EPO)对肾性贫血患者临床症状及其对红细胞膜的保护作用。方法:60例肾性贫血患者随机分为养血饮合益比奥治疗组30例(中西组),西药益比奥组30例(对照组),治疗3个月后用流式细胞仪检测CD55、CD59、ROS,并检测和评估血常规的变化。结果:实验前后。结果:对照组和中西组治疗后HB,HCT均较前明显上升(P<0.01),两组之间无明显差异。红细胞膜上CD55、CD59治疗前后无明显差异,两组间比较也无明显差异。红细胞膜上ROS均明显高于正常组;治疗后均较治疗前明显降低(P<0.01),但两组之间无明显差异。结论:中药养血饮联合促红细胞生成素治疗肾性贫血均有明显治疗效果。肾性贫血患者除促红素生成不足外,还存在红细胞寿命缩短,中西医结合治疗方案通过增加红细胞数量和减少ROS的途径保护红细胞。

Objective ： To study the effect Of combination of yangxueyin Recipe and high dosage erythropoietin （ EPO ） in the treatment of renal anemia and the influenCe on change of CD55, CD59 and ROS, so as to explore the possible mechanism of inter grative Chinese and Western medicine （ICWM） in treating renal anemia. Methods：60 patients with renal anemia were randomly divided into two groups ： the ICWM group and the control group with 30 in each group. The symptomatic and supporting treatment, such as dialysis supplementing of ferrous, foliac acid and vitamin B12,was given to both groups. 3 months later, blood levels of he- moglobin（Hb） ,hematocrit（Hct） ,CD55, CD59 and ROS were measured and the therapeutic effect was observed. Results：After treatment,levels of HB and HCT in two groups were obviously increased（P 〈0. O1 ） ibut there was on difference between the two groups. CD55 and CD59 in two groups had no difference before and after treatment and there was also no difference between the two groups. ROS level in two groups was obviously higher than that in normal group and after treatment it was decreased more sig- nificantly（P 〈0. 01 ） ,but there was no difference between the two groups. Conclusion：The control and ICWM groups both could significantly inhibit the production of ROS, and this may be an important factor for ICWM and control group in effectively impro- ving renal anemia.