铜胁迫对饲料油菜种子萌发的影响

Effects of copper stress on seed germination of Feed Brassica napus L.

以饲料油菜种子为材料,采用培养皿滤纸发芽法,进行不同浓度CuSO4胁迫下种子发芽试验(浓度梯度设0、20、40、80、160、320 mg/L),测定胚根长、胚鲜重、发芽率、发芽势、发芽指数和脂肪酶活性等,研究铜胁迫对饲料油菜种子萌发的影响。结果表明,低浓度(20、40 mg/L)铜处理能促进饲料油菜种子的萌发,种子胚根长、胚鲜重、发芽率、发芽势、发芽指数和脂肪酶活性等均明显高于对照,其中20 mg/L处理差异显著;但随着铜浓度的进一步增大,种子发芽性能较对照均有不同程度下降。低浓度铜刺激对饲料油菜种子发芽有促进作用,但高浓度铜胁迫对发芽有抑制作用。

The purpose of this study was to investigate the effects of copper stress on germination of Feed Brassica nopus L. Using Petri dish-paper germination method, seed of Feed B. napus L was treated with different concentrations （0, 20, 40, 80, 160 and 320 mg/L） of CuSO4, and the length of radicle, fresh weight of embryo, germination rate, germination energy, germination index and lipase activity were measured. The results showed that low concentrations （20 or 40 mg/L） of copper could promote seed germination, and length of radicle, fresh weight of embryo, germination rate, germination energy, germination index and lipase activity were higher than that of the control, and the effects of 20 mg/L copper was more significant. However, compared to the control, the high concentration of copper could decrease the efficiency of seed germination. Low concentrations of copper could stimulate seed germination while high concentrations of copper inhibit seed germination of Feed B. napus L.