西瓜枯萎病菌分离、鉴定及室内毒力测定

Isolation, identification and laboratory toxicity determination of Fusarium oxysporum from watermelon root rot

从西瓜根腐病病株中分离得到镰刀菌10株,经鉴定分别为尖孢镰刀菌(7株)、串珠镰刀菌(2株)、茄病镰刀菌株(1株)。其中尖孢镰刀菌的致病性较强,发病率为88.2%,其他两种菌的发病率较低,分别为28.4%和29.7%。对尖孢镰刀菌进行室内毒力试验,结果表明表明万寿菊杀菌素水乳剂与适乐时具有较好的防治效果。

In this study, ten strains were taken at the colony edge for subculture and purification from watermelon root rot. The purified isolates were primarily identified based on morphological and colony characteristics, seven species belonged to Fusarium oxysporum, three species belonged to F. moniliforme and one species belonged to F. solani. Our pathogenicity test showed that F. oxysporum was major pathogen of root rot than F. moniliforme （28.4%） and F. solani 29.7%, which pathogenic rate up to 88.2%. In addition, the toxicity of 6 fungicides to F. oxysporum was tested. The result showed that carbendazim and marigold bactericidin diplayed obvious inhibitive effects.