废丝废茧制备的氨基酸肥水剂对小球藻生长的影响

Effects of amino fat agent prepared by waste sile and cocoon on the growth of Chlorlla spp.

缫丝汰头中含有丰富的废丝废茧废弃蛋白质,废弃蛋白经酸水解后,得到的氨基酸水解液中游离的氨基酸N,可为小球藻生长提供丰富的来源。以小球藻生物量为指标,通过单因素试验研究不同浓度的氨基酸水解液、K2HPO4、MgSO4、Na2EDTA、光合细菌和芽孢杆菌对小球藻生长的影响,得到小球藻生长的最适浓度,制备出一种以废丝废茧为原料的氨基酸肥水剂,配方为:pH值7.0～7.2、浓度83.33%的氨基酸水解液10 mL,每200 mL肥水剂含K2HPO40.8 g、MgSO4.7H2O 2.0 g、Na2EDTA 0.025 g、1.02×104CFU光合细菌∶5.4×104CFU芽孢杆菌=1∶1。

Silk reeling frigon contains large amounts of waste sile and cocoon protein, through hydrolysising, we can get amino acid hydrolysate, in which the free amino acid N can provide abundant source for the growth of chlorella. Taking chlorella biomass as indicators, the paper used single factor experiment to study the effect of some factors inclding different concentrations of amino acid hydrolyzate, K2HPO4, MgSO4, Na2EDTA, photosynthetic bacteria and bacillus on the growth of chlorella. This paper prepared an amino acid fertilizer agent using waste sile and cocoon as raw material, the formula was following： 10 mL amino acid hydrolysate which pH 7.0- 7.2 and the concentration of 83.33%; 0.8 g K2HPO4, 2.0 g MgSO4 7H20, 0.025 g Na2EDTA, 1.02~104 CFU photosynthetic bacteria：5.4× 104CFU bacillus=1：1 in each 200 mL fat agent.