规模化蛋鸡养殖场大肠杆菌耐药性调查

Survey on drug tolerance to E. coli in a large scale layer-raising farm

从南宁周边某蛋鸡养殖场病死的雏鸡、5～12周龄青年鸡和蛋鸡中采集病料20份进行病原菌的分离、鉴定、动物致病性试验和血清型测定。分离出18株致病性大肠杆菌,主要有O50、O133、O88、O8、O785个血清型。用25种抗菌药物进行药物敏感性试验,结果表明,80%的分离菌株对美罗培南、亚胺培南、头孢噻肟高敏;80%的分离菌株对氨苄西林、苯唑西林、大观霉素、四环素和氯霉素产生不同程度的耐药性;100%的分离菌株对多粘菌素B、复方新诺明和链霉素耐药,说明该养殖场大肠杆菌耐药性非常严重。

This work was aimed to investigate the drug tolerance to E.coli in a large scale layer farm. 20 samples were collected from chicks, pullets in 5-12 weeks old and layers, all of which died of disease, in a large scale layer-raising farm around Nanning, Guangxi province. The samples were dealt with isolation and identification of Pathogenic Bacteria, Pathogenic Test and Serotype identification, and 18 strains collected from them were mainly blood-grouped serologically as O50, O133, O88, O8, O78. The drug sensitivity testing to E.coli of 25 drugs was done by Kirby-Bauer method. The results showed that 80% of the separated strains had a high sensitivity to meropenem, imipenem and cefotaxime while they showed various degrees of drug tolerance to 23 Ampicillin, Oxacillin, Spectinomyc, Tetracycline and ehloramphenicol. And 100% of the separated strains showed drug tolerance to polymyxin B, eotrimoxazole and streptomycin. It suggested that the drug tolerance to E-coli in the layer - raising farm was very serious.