珠海淇澳岛4种红树植物ITS区段的序列测定

Study on ITS sequences of four kinds of mangrove plants in Qiao island

以改良的CTAB法提取珠海淇澳岛4种红树植物的总DNA,使用通用引物分别对其ITS区段的序列进行PCR扩增及测序,并对所测序列进行比对分析。结果表明,桐花树、木榄、杨叶肖槿和海漆的ITS1长度分别为404、362、460、439 bp;ITS2长度分别为219、208、231、226 bp;将4种红树植物ITS区段的序列测定结果提交到GenBank,获得了登录号。

The genomic DNA of the four kinds of mangrove plants were extracted from leaves by an improved CTAB method, the ITS sequences were amplified by PCR with universal primers and the PCR products were direct sequenced. The length of the ITS1 of four kinds of mangrove plants were 404 bp, 362 bp, 460 bp and 439 bp, respectively; the length of the ITS2 were 219 bp, 208 bp, 231 bp and 226 bp, respectively. Submitted the results of the ITS sequences of four mangrove species to GenBank, then the Registry Number obtained.