栽培参及其伪品的脱氧核糖核酸指纹特征研究

Characterization of DNA Fingerprint of Cultivated Panax ginseng and Its Adulterants

目的探讨人参及其伪品的基因组特异性片段特征,建立人参与伪品的脱氧核糖核酸(DNA)指纹鉴定方法.方法采用CTAB-SDS结合法提取人参及伪品基因组DNA,紫外分光光度计检测DNA纯度,设计特异性引物,并使用该引物对人参及其伪品DNA进行PCR扩增.结果人参与伪品提取出的基因组DNA大于23 kb,其纯度为1.70±0.19,并且人参能扩增出194 bp大小的片段,而其伪品未能扩增出相应片段.结论人参DNA片段具有特异性指纹特征,可作为人参与其伪品鉴定的有效方法,方法简便,结果可靠.

Objective To explore the characterization of specific genome fragment of Panax ginseng C. A. Mey and its adulterants, and to establish an identification method of Panax ginseng C. A. Mey and its adulterants with DNA fingerprint. Method The CTAB-SDS method was used to extract genomic DNA from Panax ginseng C. A. Mey and its adulterants, the purity of DNA was detected by UV spectrophotometer,the specific PCR primers were designed, and the DNA was amplified by utilizing the primer with PCR technique. Results All DNAs obtained from the samples were greater than 23 Kb, the purity was 1.70 ± 0.19, a fragment with 194 bp could be amplified only from Panax ginseng C. A. Mey, but not from its adulterants. Conclusion DNA fragment of Panax ginseng C. A. Mey could show a specific fingerprint characteristic, suggesting that it can be used to identify Panax ginseng C. A. Mey from its adulterants. The method should be effective, simple, rapid and reliable.