系统生物学技术用于中药肝毒性发现与评价的研究进展

Research Development on the Application of Systems Biology in the Discovery and Assessment of Hepatotoxicity of TCM

目的梳理近10年来基因组学、蛋白质组学、代谢组学等系统生物学技术在中药肝毒性标志物筛选、肝毒机制研究、毒性预测与毒性蛋白质、基因组数据库建立等方面研究进展与应用前景。方法对近10年来国内外发表的相关文献进行分析、整理和归纳。结果与传统毒理学研究方法相比,系统生物学技术可更快、更准确地发现毒性物质,用于潜在的生物标记物寻找和毒靶研究,也可作为机制研究的补充和验证;但受技术限制在应用中尚存在缺乏规范的毒性评价体系、缺乏足够的基础数据等问题。结论系统生物学技术提升了中药肝毒机制的认知水平,在中药肝毒性评价、机制研究、临床诊断领域提供了一个适宜技术,尤其为肝毒性生物标记物发掘、肝毒性预警奠定了技术可行性。

Objective To summarize the application of systems biology in hepatotoxicity of TCM by reviewing related references, including the screening of toxic markers, the research of toxicity mechanisms, toxicity prediction, and the estabhshment of toxic protein and gene database. Methods Related references in recent years at home and abroad were collected, analyzed and stmmaarized. Results Systems biology technology can find out the toxic substances and the law of toxicity more quickly and accurately compared to the traditional methods. It can either be applied to the study of biomarkers and drug targets independently, or to supplement and verify the study of mechanism. Meanwhile there are still some unsolved problems of its apphcation due to technical hmJtation such as the lack of toxic assessment system and enough basic data. Conclusion The knowledge of toxic mechanism of TCM has been raised to a new level due to the application of systems biology, which is a suitable platform for the research on hepatotoxicity assessment, chnical diagnosis and hepatotoxicity mechanism of TCM, especially for discovering toxic markers and hepatotoxicity warning in advance.