重组人组织型纤溶酶原激活剂中细胞残余DNA的检测

Detection of Residual Cell DNA from Recombinant Human Tissue Type Plasminogen Activator

采用地高辛标记探针 ,以核酸分子斑点杂交技术检测重组人组织型纤溶酶原激活剂 (rt PA)中细胞残余DNA的方法。从样品中去掉蛋白提取DNA的方法能有效的避免蛋白对检测结果的干扰 ,其灵敏度和结果都能较好地达到WHO的要求。

s Residual cell DNA of recombinant human tissue type plasminogen activator(rt PA)was detected by nucleic acids dot blot with digoxigenin labed probe The method ,which DNA extracted from deproteinized sample, can avoid that protein interfere result of detection in effect, its senstivity and result can attain requirment of WHO in good