复方芪苓无糖颗粒对高脂致血管内皮细胞损伤的保护作用

Protective Effects of Sugar-free Qiling Compound Granules on Endothelial Vascular Cells Against Inju ries Induced by Hyperlipidemic Serum

目的:观察复方芪苓无糖颗粒对高脂损伤血管内皮细胞的保护作用,探讨其治疗高脂血症的作用机制。方法:用高脂血清造成人血管内皮细胞(EVC)损伤模型,以复方芪苓无糖颗粒及血脂康含药血清进行干预。光镜下观察细胞形态变化,MTT法检测细胞活性;采用逆转录-聚合酶链反应(RT-PCR)技术检测复方芪苓无糖颗粒高、中、低剂量各组对高脂血清诱导的血管内皮细胞表面黏附分子(TGF-β1)基因表达。结果:复方芪苓无糖颗粒各剂量组及血脂康组均能使高脂损伤的EVC形态趋于正常,活性增强,细胞膜损伤减轻;并均有上调内皮细胞TGF-β1的趋势,血脂康组TGF-β1表达与复方芪苓颗粒中、高浓度组比较无统计学意义(P>0.05)。结论:复方芪苓无糖颗粒对高脂损伤的血管内皮细胞有保护作用,可能是其防治高脂血症及动脉粥样硬化的作用机制之一。

Objective： To study the protective effect of sugar-free Qiling compound granules on endothelial vascu- lar cells（EVC） against injuries by hyperlipidemic serum, in an attempt to understand the underlying mechanism of sugar-free Qiling compound granules in treating hyperlipidemia. Methods： Hyperlipidemic serum was used to establish injured model of EVC. Sera with sugar-free Qiling compound granules of low, medium, high concentra- tions and Xuezhikang were applied with hyperlipidemic serum together. The morphologic changes of EVC were ob- served under light microscope and EVC activity was determined by MTT method. Reverse transcription-poly- merase chain reaction（RT-PCR） technique was used to detect hyperlipidemia induced EVC surface adhesion mole- cule（TGF-β1） gene expression in 3 Qiling groups. Results： After treatment of 3 doses of Qiling compound and Xuezhikang, abnormal morphologic changes of EVC induced by hyperlipidemic serum gradually retained to nor- mal, the activity of EVC increased, and the injury of cell membrane lessened. The mRNA＇s expression of TGF-β1 in all groups increased after treatment and the expression was not statistically different between the Xuezhi- kang group and the sugar-free Qiling groups with medium and high concentrations（P〉0.05）. Conclusion：Sug- ar-free Qiling compound granules have a protective effect on EVC against injuries induced by hyperlipidemic serum and can be used as prevention and treatment for hyperlipidemia and atherosclerosis ideal medication.