不同人群血清中TTV核酸检测

Detection of TTV DNA in sera from various populations

用巢式聚合酶链反应对成都地区 15 1例自然健康人群、 115例职业献血员、 11例血透析病人、 15例甲型肝炎、 185例急性乙肝、 116例慢性乙肝、 2 1例慢性丙肝、 8例丁型肝炎、 13例戊型肝炎和 36例非甲～戊非庚型肝炎病人血清同时检测TTVDNA的ORF1和ORF2区 ,结果在上述 10种人群中TTVDNA阳性率分别为 5 30 %、 6 96 %、 2 7 2 7%、 6 6 7%、 10 2 7%、12 93%、 19 0 5 %、 12 5 0 %、 7 6 9%和 41 6 7%。 1例急性乙肝患者ORF2引物区出现阴性 ,而ORF1检测结果为阳性。血透析、非甲～戊非庚型肝炎病人TTV感染率显著高于其他人群 (P <0 0 5 )。在TTVDNA阳性者中 ,接受过输血或血制品者占88% ,高于未接受过血或其制品者 (P <0 0 5 )。

Nested polymerase chain reaction with the primers derived from TTV ORF1 and ORF2 regions were performed to detect TTV DNA in the sera from 151 general healthy individuals,115 blood donors,11 hemodialysis patients,15 patients with hepatitis A,185 patients with acute hepatitis B,116 patients with chronic hepatitis B,21 patients with chronic hepatitis C,8 patients with hepatitis D,13 patients with hepatitis E,36 patients with non A～G hepatitis.The positive rates of TTV DNA in these populations were 5 30%,6 96%,27 27%,6 67%,10 27%,12 93%,19 05%,12 50%,7 69% and 41 67%,respectively.TTV DNA in the serum from a patient with acute hepatitis B was negtive with the primers derived from ORF2,but it was positive with the primers from ORF1.The positive rates in the hemodialysis patients and the patients with non A～G hepatitis were higher significantly than the others( P <0 05).66 of75 people with TTV DNA have histories of transfusing blood or blood products.