云南稻微核心种质及其回交高代糙米功能成分含量的遗传变异

Genetic variation of functional components in brown rice of mini core collection of Yunnan landrace rice and its advanced backcross lines

测定了作为供体的95份云南稻微核心种质、籼稻轮回亲本滇屯502、粳稻轮回亲本合系35及其籼籼交和粳粳交的95份回交高代糙米的总黄酮、γ–氨基丁酸和抗性淀粉含量,并进行遗传变异分析。结果表明:1)供体及其回交高代糙米的3种功能成分含量变幅和变异系数均较大,大小依次为抗性淀粉、γ–氨基丁酸、总黄酮;2)受体糙米的功能成分含量较低,回交高代3种功能成分含量比受体有提高,供体糙米总黄酮含量极显著高于回交高代,粳稻糙米回交高代的γ–氨基丁酸含量大于其对应的供体,供体与对应的回交高代糙米抗性淀粉含量均呈双峰分布且遗传稳定,说明抗性淀粉含量主要受基因型控制。

Ninety-five assessions from the mini core collection of Yunnan rice and their advanced backcross lines, which were obtained using the 95 accessions as donor, the Diantun 502 as the recurrent parent of Indica rice and the Hexi35 as the recurrent parent of japonica rice, were tested for their genetic variation, and the content of 3 functional components, namely, total flavonoids （TF）, γ-aminobutyric acid （GABA） and resistant starch （RS） in the brown rice were determined. The results show that （1） there are significant difference in the variation coefficients and the ranges ofFL, GABA and RS contents, with RS showing the highest variation coefficients followed by GABA and TE （2） the contents of 3 functional components in brown rice of the acceptor are low, which were increased in the corresponding advanced backcross lines, the content of total flavonoids in brown rice of the donor were significantly higher than those of the advanced backcross lines, the content of GABA in brown rice of japonica advanced backcross lines was higher than those of the corresponding donor.The content of RS in brown rice of the donor and its advanced backcross lines are bimodally distributed, with low genetic variation, indicating the the content of RS is controlled by genotype.