鱼腥草快速繁殖技术体系的建立

Established the rapid propagation technological system of Houttuynia cordata Thunb

以鱼腥草(Houttuynia cordata Thunb)的地下茎为材料,通过外植体消毒、不定芽诱导、不定芽增殖、生根和驯化移栽,建立鱼腥草快速繁殖体系。结果表明:链霉素+0.5 g/L青霉素钠浸泡12 h+70%乙醇浸泡30 s+0.1%氯化汞处理12 min+0.1%氯化汞处理10 min的灭菌效果最好;在MS中添加0.5 mg/L NAA和2.0 mg/L 6–BA最适合不定芽的萌发和生长;添加0.5 mg/L NAA和1.0 mg/L 6–BA的MS培养基均能有效促进不定芽的增殖;在MS培养基中添加4～5 mg/L GA3,能促进鱼腥草不定芽的伸长和增殖;NAA促进丛生芽生根的效果好;将再生苗移栽至用栽培土和蛭石配制的基质上,其成活率高达99%,且生长势良好。

The subterranean stems ofHouttuynia cordata Thunb were employed for the purpose of establishing the rapid propagation system using explants sterilization, adventitious bud differentiation and multiplication, rooting and transplanting approaches in the paper. Results from the research were as follows：① The best sterilization approach was to sterilize explants using streptomycin and penicillin （0.5 mg/L） for 12 hours firstly, then using HgC12 （0.1%） for 12 minutes and HgC12 （0.1%） for 10 minutes respectively; ② The best condition for the germination and growth of adventitious buds was adding 0.5 mg/L NAA and 2.0 mg/L 6-BA to MS medium, while, if 0.5 mg/L NAA and 1.0 mg/L 6-BA were added to MS medium, the propagation of adventitious buds could be effectively promoted. ③ Adding 4-5 mg/L GA3 to MS medium could promote proliferation and growth of adventitious buds; ④ NAA had a good promotional effect on improve budding and rooting ofHouttuynia cordata Thunb. The optimal transplanting medium was cultivated soil and vermiculite which could result in 99% survival rate and healthy growing regeneration seedlings.