植物检疫性有害生物蔗扁蛾的PCR快速鉴定

Rapid PCR-based identification of the plant quarantine pest Opogona sacchari (Bojer)

基于PCR分子检测技术,建立了进境植物检疫性有害生物蔗扁蛾(Opogona sacchari)快速、准确的鉴定方法。该方法对待检样品的基因组DNA经PCR扩增后进行凝胶电泳图分析,实现对待检样品的准确鉴定,检测时间缩短至8 h内,检测基因组DNA质量浓度限度为1 ng/μL,最适检测限为10 ng/μL以上。

Based on polymerase chain reaction （PCR）, a rapid and accurate identification method was established for detection of the import plant quarantine pest Opogona sacchari. Genomic DNA of the samples was used as template in PCR amplification, and the PCR products were analyzed by gel electrophoresis. By PCR amplification, the detection time for Opogona sacchari was shortened to less than 8 hours. The test limit for genomic DNA concentration was 1 ng/μL, and the concentration above 10 ng/μL was the optimal.