无机砷及其代谢物诱导人成纤维细胞凋亡的实验研究

Inorganic arsenic and its metabolites induced fibroblast apoptosis in experimental research

目的体外培养人成纤维细胞，用无机砷及其代谢物进行染毒，观察染毒后细胞凋亡的影响。方法从8例7～11岁的健康儿童志愿者中，取包皮组织分离，培养成纤维细胞，将无机砷及其代谢物作用于培养至第10代的人成纤维细胞后，通过四唑盐比色法、流式细胞仪观察无机砷及其代谢物能否诱导人成纤维细胞凋亡。结果四唑盐比色法证实，当无机砷及其代谢物为0～10μmol／L时，对人成纤维细胞有明显的增殖作用，凋亡不明显；而10～25μmol／L浓度时产生明显的毒性作用，对人成纤维细胞的凋亡率呈浓度依赖性。结论无机砷及其代谢物对人成纤维细胞有一定的毒性作用，而低浓度对人皮肤成纤维细胞有增殖效应。

Objective To observe the influence of intervention on apoptosis after human fi- broblasts were cultured in vitro and intervened with inorganic arsenic and its metabolites. Methods Foreskin tissues were taken from 8 healthy volunteer children aged from 7 to 11 years old and cultured into fibroblasts. Then inorganic arsenic and its metabolites were applied to culture the tenth generation human fibroblasts. Tetrazolium dye （MTT） eolorimetry and flow cytometry were employed to observe whether inorganic arsenic and its metabolites could induce apoptosis in human fibroblasts. Results MTT confirmed that 0 - 10 μmol/L inorganic arsenic and its metabolites had marked effect on human fibroblasts proliferation but less effect on apoptosis, and 10 -25 μ mol/L concentration generated no apparent toxic effects on the rate of apoptosis of human fibroblasts in a concentration-dependent man- ner. Conclusion A high concentration will cause cytotoxicity, while low concentrations of inorganic arsenic and its metabolites has an effect of proliferation to human skin fibroblasts.