脑缺血后脑组织诱生型一氧化氮合酶组织病理学及轻度低温对其影响

Effect of mild hypothermia on inducible nitric oxide synthase and pathological change following ischemia and reperfusion injury

目的观察脑缺血与诱生型一氧化氮合酶（ｉＮＯＳ）基因表达和组织病理的关系及轻度低温的影响。方法沙鼠３０只分假手术组、缺血组及轻度低温组（颞肌温度 ３３℃，１２ｈ），夹闭双侧颈总动脉３０ ｍｉｎ，再灌注２４、４８ ｈ检测脑组织ｉＮＯＳ基因表达及组织病理变化。结果原位杂交显示缺血组胶质细胞等呈现ｉＮＯＳ基因表达，海马区ＣＡ１神经细胞部分皱缩及缺失，轻度低温组与缺血组相比ｉＮＯＳ阳性细胞明显减小，灰阶值也有明显下降（Ｐ＜０．０１），神经元结构基本正常。结论轻度低温抑制脑缺血后脑组织ｉＮＯＳ 基因表达及减轻海马区组织损害程度可能是其脑保护机制之一。

Objective To observe the effect of mild hypothermia on inducible nitric oxide synthase (iNOS)and pathological change following cerebral ischemia and reperfusion injury. Methods 30 gerbils were divided into 3 groups:Sham - operated,ischemia, mild hypothermia (temporal muscles remain 33℃, 12h). Cerebral ischemia last 30 win by occluding bilateral common carotid and arterial and reperfuction for 24 h,48 h. INOS gene expression was detected by the situ hybrization and pathological change was observed by HE. Results INOS gene expression in ischemia group was presented in glial cell etc.Compared with is- chemia grovp, mild hypothermia induced down regulation of iNOS gene expression and decreased the pathological change of postischemic pyramidal neurons.Con- clusion Mild hypothermia which inhibited the iNOS gene expression and reduced the extent of pathological change may be one of the protective mechanisms on cerebral ischemia..