檀香叶中的γ-氨基丁酸的HPLC测定研究

Determination of γ-Aminobutyric Acid in Leaves of Santalum album L.by HPLC

建立了檀香叶中γ-氨基丁酸(GABA)含量的高效液相色谱检测方法。以乙醚为溶剂,采用索氏提取除去色素,水提醇沉去除糖类和蛋白质的净化方法,以丹酰氯为衍生剂,高效液相色谱法分离,荧光检测器测定,外标法定量。峰面积与浓度之间线性关系良好,回归方程为y=475 211 x+69 037,R2=0.999 8,加标回收率为112%～120%,检测限为7μg/g。该方法操作步骤简单,灵敏度高,用于檀香叶中GABA的检测,方法简便、准确、高效。

A method was established by reversed-phase high performance liquid chromatography for determining the content of γ- aminobutyric acid （GABA） in leaves of Santalum album L. The sample was pretreated by soxhlet extraction to eliminate the pigments with ether, and then by water extraction and alcohol precipitation to eliminate sugar and protein. GABA was derivatized with dansyl chloride （Dns-Cl） and isolated by reversed-phase HPLC, GABA was detected quantitatively by HPLC with fluorescence detection. Nice linear relationship between peak area and concentration was obtained with the linear regression equation as y =475 211x ＋69 037 ,R^2 =0. 999 8. The recovery of standard addition was in the range of 112% to 120%. The detection limit of GABA was 7 μg/g. The established HPLC method is simple, economic, stable and accurate with good reproducibility. It can be applied on determination of GABA in leaves of Santalum album L.