摘要
目的建立测定散结止痛颗粒中芍药苷含量的方法。方法用高效液相色谱法测定芍药苷含量。采用依利特HypersilODS2(4.6×250mm,5μm)色谱柱,以乙睛—水(17:83)为流动相,流速:1.0ml/min,检测波长:230nm。结果芍药苷在52.4～524μg/ml范围内呈较好线性关系,r=0.9999;该方法具有良好的精密度、稳定性和重复性,平均回收率99.76%,RSD4.68%。结论此法准确可靠,适用于测定芍药苷的含量,可用于散结止痛颗粒的质量控制。
Objective To establish a method for the determination of paeoniflorin in Sanjie Zhitong granule. Methods The content of paeoniflorin was determined by HPLC. To use the Hypersil ODS2 column （4. 6 × 250 ram, 5μm） ,the mobile phase was acetonitrile-water（ 17：83 ）, the flow rate was 1.0 ml/min, the detection wavelength was 230 nm. Results The linear range of paeoniflorin was within 52. 4 - 524 μg/ml （ r = 0. 9999）. The average recovery was 99. 76% （ RSD=4. 68% ）. Conclusion The method is accurate and reliable. It can be applied in the determination of paeoniflorin in Sanjie Zhitong granule.
出处
《环球中医药》
CAS
2013年第3期172-174,共3页
Global Traditional Chinese Medicine
