慢性ITP患者外周血NO/NOS、ROS的检测及临床意义

The detection and clinical significance of peripheral blood NO/NOS and ROS in patients with chronic ITP

目的:检测慢性特发性血小板减少性紫癜(Idiopathic thrombocytopenic purpura,ITP)患者血清一氧化氮(NO)、一氧化氮合酶(NOS)、活性氧(ROS)含量,探讨NO与ROS、NOS与ROS之间的相关性。方法:设立慢性ITP患者组与健康对照组,ELISA法检测外周血中NO及NOS的表达,ELISA法检测外周血ROS浓度。结果:慢性ITP患者组NO/NOS与ROS水平明显高于正常对照组[t(ROS)=1.24;t(NO)=0.91;t(NOS)=0.82,P均<0.01],ROS与NO/NOS水平分别正相关(r=0.817,r=0.861,P均<0.01)。结论:慢性ITP患者NO/NOS、ROS均升高且NO/NOS与ROS呈正相关,提示NO可能作为一种介质和自由基参与血小板的破坏,其作用在ITP发生中可能具有与ROS同样或协同的作用,NO/NOS、ROS的检测对慢性ITP的诊治具有潜在的临床指导意义。

Objective：To detect the serum NO/NOS, ROS content from the patients with chronic ITP, and explore the relationship between NO/NOS and ROS respectively. Method：Chronic ITP patients and healthy control group were set in this study, peripheral blood NO and NOS expression were detected with the ELISA assay, peripheral blood ROS concentrations was detected with ELISA assay also, then analyzed the correlation bewteen the NO/NOS and ROS. Results： NO/NOS and ROS levels in patients with chronic ITP group were significantly higher than the control group It （ROS） = 1.24; t （NO） = 0.91; t （NOS） = 0.82,all P〈 0. 01 ], ROS and NO/NOS levels were positively correlated （r = 0. 817, r = 0. 861, all P 〈0.01）. Conclusion：Elevated ROS may have a certain relationship with the destruction of megakaryocytes and platelets in patients with chronic ITP, elevated NO/NOS and positively correlated with ROS suggests that NO may be a medium and radicals involved in the destruction of megakaryocytes and platelets, and they may have a synergistic effect with ROS. The detection of NO/NOS and ROS may have clinical significances in the diagnosis and treatment of chronic ITP.