不同尿标本视黄醇结合蛋白质和甲状腺功能相关激素的比较

Comparison of retinol-binding protein and thyroid function related hormones of different urine specimens

目的比较分析晨尿和随机尿中视黄醇结合蛋白质(RBP)、促甲状腺激素(TSH)及游离甲状腺激素的水平,拓展以随机尿替代晨尿的应用领域。方法随机选取12名门诊志愿者,采集其晨尿和随机尿,离心、留上清液。测定尿液的TSH、游离三碘甲腺原氨酸(FT3)、游离甲状腺素(FT4)及尿肌酐(CR);提取尿蛋白、裂解、离心、测定提取的总蛋白水平,行Western blot并对其条带进行光密度扫描。结果经Cr校正后,晨尿和随机尿TSH、FT3和RBP水平的差异无统计学意义(t=0.903,P>0.05;t=1.217,P>0.05;t=1.880,P>0.05);晨尿和随机尿FT4水平的差异具有统计学意义(t=2.436,P<0.05)。结论晨尿和随机尿RBP、TSH及FT3水平没有明显差异,可以用随机尿替代晨尿来进行这些项目的检测和研究,方便尿液标本的留取,提高尿液标本的实用性。

Objective To comparative analyze the contents of the retinal-binding protein（RBP） ,thyrotropin（TSH） and free thy-roid hormone in the urine sanguinis and the random urine, and to explore the alternative of urine sanguinis with random urine. Methods Urine sanguinis and random urine were collected from 12 outpatient volunteers. After centrifugation,supernatant of urine was tested for TSH, free triiodothyronine （FT3）, free tetraiodothyronine （FT4） and urine ereatinine （Cr）. Urine proteins were ex-tracted and dissociated. After centrifugation,levels of extracted proteins were determined. Then Western blot was employed and the optical density of bands was scanned. Results After correction with Cr, there were no statistical significances between urine sangui-his and random urine for TSH,FT3 and RBP（t=0. 903,P〉0.05;t=1. 217,P〉0.05;t=1. 880,P〉0.05）. There were significant differences of FT4 between urine sanguinis and random urine（t=2. 436,P〈0.05）. Conclusion There are no statistical signifi-cances between urine sanguinis and random urine for RBP,TSH and FT3. Random urine could be an alternative of urine sanguinis for convenient availability and applicability for these tests.