肺鳞癌组织中人乳头状瘤病毒DNA的分型检测及临床意义

Typing of HPV DNA and its clinical significance in lung squamous cell carcinoma

目的探讨河南漯河周边地区人乳头状瘤病毒(HPV)各型在肺鳞癌中的感染情况及其与临床病理参数之间的关系。方法采用核酸分子快速导流杂交基因芯片技术(HybrMax)检测60例新鲜肺鳞癌组织和15例癌旁正常组织中各型HPVDNA的感染情况。结果 HPV DNA在肺鳞癌组织中的感染率为38.3%高于癌旁正常组织中的感染率6.7%,差异有统计学意义(P<0.05);HPV DNA在肺鳞癌组织中的感染与患者性别呈正相关(r=0.272),与肿瘤组织的分化程度呈负相关(r=-0.266),和患者的年龄,肿瘤的大小,以及淋巴结转移无关(P>0.05)。结论 HPV感染与肺鳞癌的发生、发展可能具有病因学关系。

Objective To investigate the relationship between the human papillomaviruses（HPV） infection and clinicopatholo gic features in lung squamous cell carcinoma（SCC） on surrounding areas of Luohe. Methods Flow through hybridization and gene chip （HybrMax） was used to detect the various HPV infection in 60 case of lung SCC and 15 case of paraneoplastic tissues. Results HPV infection in lung SCC was significant higher than that in surrounding areas（P〈0.05）, and HPV infection was negatively correlation with histological type, degree of SCC differentiation（r=0.266）, and correlation with gender of the patient（r=0. 272）. There was no correlation with the age of the patient,the size of the tumor,and lymph node status（P〉0.05）. Conclusion There was perhaps eti-ologic relationship between HPV infection and the occurrence of lung SCC.