摘要
目的探讨在SD大鼠肝细胞癌中Shh和Gli1蛋白的表达及其意义。方法取体质量为170～200g雄性SD大鼠30只,随机分为实验组(20只)和对照组(10只),实验组采用0.01%二乙基亚硝胺(DEN)自由喂养16周,对照组常规标准喂养,诱导大鼠肝细胞癌成功后,应用免疫组化检测肝癌组织和正常肝组织中Shh和Gli1表达情况。结果免疫组化检测实验组肝癌组织中Shh和Gli1的阳性表达率分别为87.5%(14/16)和75.0%(12/16),对照组肝组织中检测Shh和Gli1的阳性表达率分别为30.0%(3/10)和20.0%(2/10);两种蛋白质在肝癌组织中的表达与正常组织中的表达比较,差异有统计学意义(P<0.01)。结论在人工诱导的SD大鼠肝癌模型中,Hedgehog信号通路处于激活状态。
Objective To study the protein expression and significances of Shh and Glil protein in hepatocellular carcinoma in Spraque-Dawley（SD） rats. Methods Thirty male SD rats,weighing 170 to 200 g,were randomly divided into experimental group （20） and control group （10）. The experimental group rats were established diethylnitrosamine-induced （0.01 % ） hepatocarcinoma models for 16 weeks,at the same time,the control group was established with standard feeding. After the rats hepatocellular carci noma were successful induced,the expression of Shh and Glil proteins were tested in the experimental group and the control group by immunohistochemical staining. Results The positive rates of Shh and Glil protein expression in experimental group were 87.5% （14/16） and 75.0%（12/16）,and those in control group were 30.0%（3/10） and 20.0%（2/10）,respectively. The results showed significant difference of protein expression between hepatocarcinoma tissue and normal tissue（P〈0.01）. Conclusion The hedgehog signaling is abnormally activated in artificially induced SD rats hepatoearcinoma.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第12期1382-1384,共3页
Chongqing medicine
