慢性荨麻疹患者血清IL-35水平检测分析

Analysis of IL-35 in serm from patients with chronic urticaria

目的探讨IL-35在慢性荨麻疹患者致病机制中的作用。方法根据自身血清皮肤试验(ASST)将慢性慢性荨麻疹患者分成两组,应用ELISA法检测两组患者血清IL-35、IL-23浓度,流式细胞仪检测患者外周血细胞免疫功能,同时设立对照组进行比较。结果 53例慢性荨麻疹患者中,33例患者ASST测试结果为阳性,20例为阴性;两组患者与对照组比较血清IL-35浓度均升高,IL-23浓度降低(P<0.01);外周血CD8+细胞降低(P<0.05);B细胞降低,差异有统计学意义,其中ASST阳性组B细胞降低更为明显。结论慢性荨麻疹患者机体可能存在Th17/Treg(调节性T细胞)不平衡,导致病情反复迁延。

Objective To explore the significance of the detection of serum level of IL-35 for pathogenic mechanisms in the pa- tients with chronic urticaria. Methods 3 patients with chronic urticaria based on the ASST test results were divided into positive and negative groups. ELISA assay in CU IL-23, IL-35 content,and the flow cytometry was used to detect T lymphocyte subsets, NK cells,B cells,while the establishment of normal control group. Results Of the 53 patients in patients with CU,33（62.26%） were positive for ASST, 20（37.74 %）were negative for ASST. Serum levels of IL-35 in patients with CU were significantly higher than the control normal group,and IL-23 level was lower（P〈0.01）. The percentage of CD8＋ .13 cells were lower in the patients with CU,and the ratio of CD4＋/CD8＋ was higher（P^0.05）. B cells decreased, the difference was statistically significant, in which ASST positive group was lower in B cells. Conclusion The imbalance of Thl7/Treg（Regulatory T-cells Treg） was probably one of the immunical pathogenesis with CU.