112例自然流产夫妇染色体核型分析

Karyotype analysis on 112 cases of spontaneous abortion

目的通过对自然流产夫妇进行外周血染色体核型分析,探讨自然流产与染色体异常的关系。方法收集川北医学院附属医院产科门诊112例自然流产夫妇(共56对)的临床资料,抽取静脉血进行外周血淋巴细胞培养,常规制备染色体及G显带,分析染色体核型。结果 112例标本中有11例为染色体异常(均为单方),检出率9.82%,包括染色体平衡易位3例、罗伯逊易位1例、9号染色体臂间倒位4例及染色体多态性3例,分别占异常染色体的27.27%、9.09%、36.37%、27.27%。结论染色体异常是导致自然流产的重要原因,临床上应对不明原因的自然流产夫妇双方皆进行染色体检查,以明确病因,指导优生优育。

Objeetive To explore the correlate between spontaneous abortion and chromosome dysfunction through karyotype a- nalysis on couples. Methods 112 cases（total 56 couples） who experienced spontaneous abortion were recruited from the Affiliated Hospital of North Sichuan Medical College. Clinical data were collected. Chromosomes from cultured peripheral blood lymphocytes were analyzed using Giemsa Trypsin Giemsa（GTG）banding method. Results There were 11 cases with abnormal chromosomes a- mong the 112 cases（positive rate 9.82 % ）, including 3 balanced translocation, 1 Robertsonian translocation, 4 pericentric inversion of chromosome 9 and 3 chromosome polymorphism. The ratio among abnormal chromosomes was 27.27% ,9.09%,36.37% ,27. 27% respectively. Conclusion The dysfunction chromosome is an important reason which cause spontaneous abortion. In order to pro- vide aristogenesis instruction the clinicians should provide chromosome examination for the couples who experienced unexplained spontaneous abortion.