HER-2/neu基因在肝癌高发区原发性肝癌表达和意义

Clinical significance and expression of HER-2/neu gene in PHC patients in high incidence hepatocellular carcinoma

目的分析HER-2/neu基因在肝癌高发区厦门市同安区原发性肝细胞癌(PHC)中的表达及其与临床病理特征和预后的关系。方法应用荧光定量PCR技术检测53例原发性肝癌HER-2/neu的扩增,免疫组化对照,分析HER-2/neu的表达与临床病理特征及预后的相关性。结果在53例肝癌中有15例HER-2/neu表达,占28.3%,其中高拷贝扩增有2例(3.77%),中拷贝表达有4例(7.54%),低拷贝表达有9例(17.0%),HER-2/neu的表达与性别、年龄、AFP水平、HBV-DNA比较差异无统计学意义(P>0.05),而与肿瘤大小、病理类型、生存期(术后1年)、癌旁组织比较差异有统计学意义(P<0.05);HER-2/neu在肝硬化及正常肝组织中的表达率分别为8.3%(1/12)和无表达。结论 HER-2/neu基因在PHC的生长和转移中可能具有一定的促进作用,可作为PHC的预后指标。

Objective To investigate the expression of HER-2/neu gene in patients of primaty hepatocellular carcinoma（PHC） in high incidence hepatocellular carcinoma in Tongan of Xiamen and its relationships with clinicopathological parameters and prog- nosis. Methods 53 surgical samples from patients with primary PHC were detected for their HER-2/neu gene by FQ-PCR tech-nique,and then the correlations between expression of HER-2/neu and clinicopathological characteristics,prognosis were analyzed. Results HER-2/neu gene of 53 surgical samples from patients with primary PHC was detected, 15 of 53 （28. 30/oo） primary PHC, ,. including 2（3.77%） cases with high copies and 4（7.54%） with middle copies,9（17.0%） with low copies. It was signifi-cantly difference after 1 year survival time, tumor size, type of PHC, histological grade and Adjacent tissues with expression of HER-2/neu of PHC patients（P〈0.05） but no to sex,age,AFP level, HBV-DNA copies of PHC patients（P〉0.05）. ,expression of HER-2/neu in cirrhosis and normal liver tissue were 8.3%（1/12） and free expression. Conclusion It was found that there were a expression of HER-2 gens might be a involved factor in the development and proliferation of PttC patients ,and seemed to be a val-uably independent prognosis index predicting postoperative survival of patients with PHC.