陆地棉GhBI-1基因在拟南芥中的异位表达及耐盐性研究

Ectopic Expression of GhBI-1 Gene from Upland Cotton in Arabidopsis thaliana and Study on Its Salt Tolerance

前期工作中,我们从陆地棉基因组中分离了GhBI-1基因全长cDNA,构建了GhBI-1过量表达载体并转化拟南芥。本研究在此基础上,利用卡那初步筛选获得转基因拟南芥,通过PCR进一步验证,得到具有卡那抗性并且遗传稳定的T3代纯合株系。利用不同浓度NaCl处理野生型和转GhBI-1基因拟南芥,结果表明,转基因拟南芥在200 mmol/L NaCl胁迫后,萌发率和生长情况要好于野生型拟南芥,过量表达GhBI-1基因能够提高拟南芥耐受盐胁迫的能力。

In our previous work, a full length cDNA of GhBI - 1 was isolated from upland cotton （ Gos- sypium hirsutmL. ）. Based on this, the GhBI - 1 gene was inserted into the plant expression vector pBI121 downstream 35S promoter and introduced into Arabidopsis thaliana by Agrobacterium tumefaciens through floral dip method. The T3 independent homozygous transgenic lines were obtained through kanamycin screening and confirmed by PCR. The salt tolerance survey was conducted by tieating transgenic plants and wild type with different concentrations of NaC1. The results showed that the germination rate and plant growth of transgenic plants were better than those of wild type plants under 200 mmol/L NaC1. It indicated that overexpression of GhBI- 1 could improve the salt tolerance of Arabidopsis thaliana.