高压氧对大鼠脊髓损伤后移植神经干细胞增殖分化的影响

Effect of hyperbaric oxygen therapy on proliferation and differentiation of transplanted neural stem cells of rats with spinal cord injury

目的:探讨高压氧(HBO)对大鼠脊髓全横断损伤后移植的神经干细胞存活、增殖及分化的影响。方法:成年健康SD大鼠20只,随机分为神经干细胞移植组(NSCs组)和HBO联合神经干细胞移植组(HBO+NSCs组)各10只。2组均采用脊髓全横断损伤模型,术后均给予NSCs移植治疗,HBO+NSCs组在移植后辅以HBO治疗。治疗后检测移植在脊髓损伤处的神经干细胞存活、增殖及分化的情况。结果:术后第28d,HBO+NSCs组移植的神经干细胞存活数量显著多于NSCs组(P<0.05)。2组大鼠的脊髓损伤处及其相邻的组织均可观察到较多移植的神经干细胞分化为胶质纤维酸性蛋白(GFAP)阳性染色细胞。HBO+NSCs组中移植的神经干细胞分化为神经元特异性烯醇化酶(NSE)阳性染色细胞百分率显著高于NSCs组(P<0.05)。结论:HBO能够促进大鼠脊髓损伤处移植的神经干细胞存活、增殖,并能促进这些细胞能分化为NSE阳性神经元。

Objective： To explore the effects of hyperbaric oxygen （HBO） therapy on the survival,proliferation and differentiation of neural stem cells （NSCs） transplanted in the injured site of spinal cord transaction in rats. Meth- ods：Twenty adult SD rats were divided into NSCs transplantation group （NSCs group） and HBO therapy plus NSCs transplantation group （HBO＋ NSCs group）. T10 spinal cord segments of all animals were completely transected. The rats in HBO-J-NSCs group and NSCs group were treated with neural stem cells transplantation after operation, and the rats in HBO＋NSCs group received HBO treatment additionally. The spinal cords were taken for observing the survival, and differentiation of neural stem cells transplanted in the injured site of spinal cord transection after 28 days in rats. Results：The surviving number of transplanted NSCs in HBO＋NSC group was more than that in NSCs group. In many transplanted NSCs at injured site of transected completely spinal cord and neighboring tissue,glial fi- brillary acidic protein （GFAP） positive staining was observed in HBO-I-NSCs group or NSCs group. The percentage of neuron-specific enolase （NSE） positive staining cells differentiated from transplanted NSCs in HBO＋ NSCs group was higher than that in NSCs group. Conclusion： HBO may prolong the survival of NSCs transplanted in the injured site of rat spinal cord transected completely, and can promote the differentiation of these cells into NSE positive cells.