头穴透刺对急性脑缺血大鼠海马齿状回nestin影响的研究

Effect of scalp-point penetration needling on the expression of nestin in hippocampal dentate gyrus of rats with acute cerebral ischemia

目的:通过观察头穴透刺对急性脑缺血大鼠海马齿状回Nestin表达的变化,探讨头穴透刺抗脑缺血损伤的作用机理。方法:雄性SD大鼠32只,随机分为正常组、脑缺血组、假手术组、头穴透刺组各8只。采用改良线栓法制作大鼠大脑中动脉栓塞局灶性脑缺血模型,头穴透刺组大鼠待清醒后1h,取百会透前顶、率谷透悬厘进行针刺治疗。治疗后对各组大鼠进行神经功能缺损评分,采用免疫组化法检测海马齿状回神经巢蛋白(nestin)的表达。结果:治疗7d后,头穴透刺组大鼠神经缺损评分明显低于较脑缺血组(P<0.01);脑缺血组与正常组及假手术组比较,nestin表达明显增加(P<0.01);与脑缺血组比较,头穴透刺组nestin阳性表达明显增加(P<0.01)。结论:头穴透刺可以使急性脑缺血大鼠受损神经功能明显恢复,能使脑缺血大鼠海马齿状回nestin的表达明显增加,提示头穴透刺有显著抗脑缺血损伤的作用。

Objective： To investigate the effect of scalp-point penetration needling on the expression of nestin in hippocampal dentate gyrus of rats with acute cerebral ischemia,and the mechanism for scalp-point penetration nee- dling resisting the injury of acute cerebral ischemia. Methods：All 32 SD rats were randomly divided into four groups, with 8 rats in each group： normal group, sham-operated group, cerebral ischemia group and scalp-point penetration needling group. Focal cerebral ischemia model of rats was made by middle cerebral artery occlusion using a thread block technique. The rats in scalp-point penetration needling group were subjected to electro-acupuncture （EA） at Baihui （GV20） through Qianding （GV21） and Shuaigu （GB8） through Xuanli （GB6） with G6805-Ⅱelectro-acu- puncture instrument after the rats woke up. After treatment,the neurologic deficit score was recorded and nestin in hippocampal deneate gyrus was detected by using immunohistochemistry. Results： After treatment for 7 days, the neurologic deficit score in scalp-point penetration needling group was obviously higher than in cerebral ischemia group （P〈0.01）. As compared with sham-operated group and normal control group,the expression of nestin in hip- pocampal dentate gyrus of scalp-point penetration needling group was increased greatly （P〈0. 01）. As compared with cerebral isehemia group, the expression of nestin in scalp-point penetration needling group was significantly in- creased （P〈0.01）. Conclusion： The neurologic impairment of rats with cerebral ischemia was improved obviously and the expression of nestin in hippocampal dentate gyrus increased by scalp-point penetration needling,indicating that scalp-point penetration needling can resist cerebral ischemia injury. [Key words]