文蛤酶解多肽对体外培养人肝癌细胞HEPG2的抑制作用

Effect of Enzymolysis Peptide Mercenene from Meretrix Meretrix Linnaeus on Proliferation of Human Hepatocarcinoma HEPG2 Cells

研究了文蛤酶解多肽对体外培养的人肝癌HEPG2细胞的抑制作用,结果表明,经50.0 g/mL文蛤酶解多肽作用后,HEPG2细胞生长受到强烈抑制,抑制率高达88.5%,其群体倍增时间延长3.9倍;经文蛤酶解多肽处理后,HEPG2癌细胞不仅外部形态发生明显改变,而且在细胞周期上也表现变化,G0/G1期细胞增多,S期和M期细胞减少并出现凋亡峰,凋亡率为9.29%.结果说明,文蛤酶解多肽可通过改变肝癌细胞HEPG2的形态结构及影响其细胞周期移行,而对体外培养的肝癌细胞的增殖活动产生比较明显的抑制作用.

Enzymolysis peptide （named as Mercenene） was preparated from Meretrix meretrix Linnaeu and used to treat the human hepatocarcinoma of HEPG2 cells to investigate its effects on proliferation, microstructure, and cell-cycle. The cell proliferation and the inhibition rates were determined by cell counting methods and MTT methods, r^spectively, the microstructures of the cells were observed by scan electron microscope, the cell cycle was analyzed by flow cytometry. The results showed that the HEPG2 cells, after it was treated by 50.0 Iw,/mL of Mercenene, grew slower than the control and the inhibitory rate of cell growth was determined to be 88.5%, and their doubling time were postponed 3.9 times to the control. Furthermore, by flow cytometry analysis methods, it was found that after they were treated by Mercencne, HEPG2 cell proportions in G0/G1 phases were increased markedly but those in the S and M phase were decreased significantly, and in the mean time, the apoptosis rate of HEPG2 cells was up to 9.29%. These results indicated that by the way of acting as an apoptosis inducer via inducing morphological changes and cell cycle arresting, Mercenene could effectively inhibit the proliferation of the human hepatocarcinoma cell line HEPG2 in vitro.