湖南省猪链球菌的病原生物学特征分析

Pathogenic Biological Characteristics of Streptococcus suis in Hunan Province

目的对5株不同年份分离的猪链球菌进行病原学特征分析,以评估其对公共卫生的危害。方法对分离菌株进行毒力相关基因检测、脉冲场凝胶电泳分析(PFGE)。结果所有菌株的相关毒力因子cps2A、map、sly和ef均为阳性。酶切图谱显示2006、2007年菌株图谱完全一致,2010年分离菌与2006、2007年分离株存在差异。结论分离菌株有相当强的致病性,从人和猪分离到菌株酶切图谱完全一致,显示为统一来源或者同一克隆群;不同地域分离的菌株酶切图谱相似度较低。

Objective To analyze the pathogenic biological characteristics of 5 strains of Streptococcus suis isolated during 2006 and 2010 in Hunan province, so as to assess its threat to public health. Methods The virulence related genes of the isolated strains were detected and analyzed by pulse field gel eleetrophoresis analysis （PFGE）. Results The virulence factors, cps2A, map, sly and el, were detected in all of the isolated strains. The restriction enzyme maps of the strains isolated in 2007 and 2006 were entirely consistent, while both were different from those isolated in 2010. Conclusions The isolated strains of Streptococcus suis in Hunan Province during 2006 and 2010 have strong pathogenicity. The restriction enzyme maps of strains from human and swine are entirely consistent, suggesting both stains have the same source or belong to the same clone population. The restriction enzyme maps of strains from different areas have a low similarity.