弥漫性大B细胞淋巴瘤免疫表型分类与c-MYC重排的相关性研究

Research on correlation between immunophenotype and c-MYC rearrangement in diffuse large B-cell lymphoma

目的探讨弥漫性大B细胞淋巴瘤(DLBCL)免疫表型分类与c-MYC重排的相关性。方法根据淋巴瘤细胞免疫标志物CD10、B细胞淋巴瘤原癌基因-6(BCL-6)及多发性骨髓瘤原癌基因-1(MUM-1)的表达情况,将46例DLBCL分为生发中心B细胞样(GCB)与非生发中心B细胞样(non-GCB)2类,用荧光原位杂交(FISH)技术检测GCB类与non-GCB类中c-MYC重排情况。结果 46例标本中共有4例(8.7%)发生c-MYC重排,其中20例GCB类中发现4例(20.0%)c-MYC重排,而26例non-GCB类中未发现c-MYC重排。GCB类和non-GCB类中c-MYC重排差异有统计学意义(P<0.05)。结论 GCB类DLBCL患者c-MYC重排发生率较高,c-MYC重排是一部分GCB类DLBCL预后差的原因。FISH技术能快速、准确、灵敏地检测出c-MYC重排。

Objective To investigate the correlation between immunophenotype and c-MYC rearrangement in diffuse large B-cell lymphoma （DLBCL）. Methods According to the lymphoma cell immune marker CD10, B cell lymphoma 6 （ BCL-6 ） and multiple myeloma oncogene 1 （ MUM-1 ） expression levels ,46 cases of DLBCL were classified into germinal center B cell-like （GCB） group and non-germinal center B cell-like （non-GCB） group, c-MYC rearrangement status was analyzed by fluorescence in situ hybridization （FISH） in GCB and non-GCB groups. Results Of the 46 DLBCL eases,4 cases （8.7%） had c-MYC rearrangement. The 4 cases of c-MYC rearrangement belonged to the GCB group. There was no c-MYC rearrangement in non-GCB group. The c-MYC rearrangement had statistical significance between GCB and non-GCB groups （ P 〈 0.05）. Conclusions The GCB group in patients with DLBCL has a high c-MYC rearrangement, and c-MYC rearrangement is a poor predictor for a part of GCB group. FISH is a rapid, accurate and sensitive technique for the detection of c-MYC rearrangement.