慢性乙型肝炎患者IFN-γ、IL-10和CD19^+水平检测与病毒载量的关系

The relationship of IFN-γ,IL-10 and CD19^+ levels with viral load in chronic hepatitis B patients

目的观察慢性乙型肝炎患者免疫功能的变化,探讨患者干扰素γ(IFN-γ)、白细胞介素10(IL-10)和CD19+与乙型肝炎病毒(HBV)DNA载量的关系。方法采用酶联免疫吸附试验(ELISA)和流式细胞术检测76例慢性乙型肝炎患者和30名正常人血清IFN-γ、IL-10和外周血CD19+;用荧光定量聚合酶链反应(PCR)检测慢性乙型肝炎患者血清HBV DNA拷贝数。结果 HBV DNA低拷贝组、高拷贝组的IFN-γ与HBV DNA阴性组比较明显下降,IL-10、CD19+与HBV DNA阴性组比较明显增高;IFN-γ水平与HBV DNA载量呈负相关(r=-0.311,P<0.05),IL-10和CD19+的水平与HBV DNA载量呈正相关(r=0.350,P<0.05;r=0.321,P<0.05)。结论 HBV感染者存在免疫功能调节紊乱,IFN-γ、IL-10和CD19+水平的检测可作为乙型肝炎相关疾病炎症活动的观察指标。

Objective To observe the change of immune function in patients with chronic hepatitis B, and to investigate the relationship of interferon-gamma （ IFN-γ ）, interleukin 10 （ IL-10 ） and CD19^＋ with hepatitis B virus （HBV） DNA load. Methods By enzyme-linked immunosorbent assay （ELISA） and flow cytometry, the serum IFN-γ, IL-10 and peripheral blood CD19^＋ levels of 76 patients with chronic hepatitis B and 30 healthy subjects were determined. By fluorescence quantitative polymerase chain reaction （PCR）, serum HBV DNA copy number was determined. Results The IFN-γ levels in HBV DNA low copy group and high copy group decreased significantly compared with those in HBV DNA negative group. Their IL-10 and CD19^＋ levels increased significantly compared with those in HBV DNA negative group. The IFN-γ levels were correlated negatively with the HBV DNA load （r = -0. 311, P 〈 0.05 ）, and the IL-10 and CD19^＋ levels were correlated positively with the HBV DNA load （ r = 0. 350, P 〈 0. 05 ; r =0. 321, P 〈 0.05）. Conclusions The patients have immune function adjustment disorder. The IFN-γ, IL-10 and CD19^＋ levels can be monitoring indices for the inflammatory of hepatitis B related diseases.