沙巴棕提取物对肺癌细胞增殖抑制及促凋亡的实验研究

Experimental study of effects of S. repens extract on lung carcinoma cell proliferation inhibition and apoptosis-promoting

目的观察沙巴棕提取物与人肺癌细胞增殖和凋亡之间的关系,并探索其作用机制。方法应用MTT比色法、流式细胞仪法观察人类肺癌细胞成活、凋亡情况。应用Western blot法观察PARP、CASP3、P27、CDKN1B、p-STAT3蛋白的表达情况。结果沙巴棕提取物以剂量依赖性的方式抑制了肺癌细胞的增殖,并通过上调PARP、CASP3、P27等凋亡相关蛋白促进凋亡。沙巴棕提取物降低了p-STAT3水平;STAT3磷酸化的一种选择性抑制剂———AG490,同样通过降低p-STAT3的水平而抑制了肺癌细胞的生长。相比于以上二者任意一种的单独治疗,沙巴棕提取物和AG490的联合治疗显著降低了肿瘤细胞的增殖(P<0.05)。结论沙巴棕提取物可通过促进凋亡信号和下调p-STAT3蛋白而抑制肺癌细胞增殖。这些观察可能为沙巴棕提取物诱导肿瘤生长抑制的机制研究提供有价值的参考。

Objective To investigate the association between S. repens extract treatment and proliferation and apoptosis of human lung cancer cells, in order to explore the potential mechanism. Methods Cell proliferation of H520 and A549 human lung cancer cells were measured by MTT assay. Cell apoptosis was assessed by flow cytometry. The protein expressions of PARP, CASP3, P27, CDKN1B and p-STAT3 were measured by Western blot. Results S. repens extract suppressed the proliferation of H520 and A549 cells in a dose-dependent manner. S. reperts extract promoted the apoptosis of glioma cells by up-regulating expression of apoptosis-related proteins, including PARP, CASP3 and P27. Moreover, S. repens extract reduced the level of p-STAT3. A selective inhibitor of STAT3 phosphorylation, AG490, also suppressed lung cancer cell growth by downregulating p-STAT3 expression. The combined administration of S. reperts extract and AG490 significantly decreased cell proliferation compared with either treatment alone （P 〈 0.05）. Conclusion S. repens extract may suppress lung cancer cell proliferation by promoting apoptosis signaling and down-regulating p-STAT3. These observations may provide valuable reference into the mechanism study of S. repens extract-induced tumor growth inhibition.