补肾健脾益气法对酒精性骨质疏松大鼠小肠中VDR-mRNA表达影响的研究

Effect of Reinforcing Kidney and Invigorating Spleen on the VDR-mRNA Expression of AOP Rats

目的:观察补肾健脾益气法对酒精性骨质疏松(AOP)大鼠小肠组织内维生素D受体(VDR)mRNA表达的影响,从基因分子水平探讨AOP发病机制及补肾健脾益气中药防治AOP的作用机理。方法:清洁级成年雄性SD大鼠40只,按随机数字表法分为模型组、西药对照组、中药干预组、正常对照组,每组各10只,用白酒灌胃法造模(空白对照组给予生理盐水灌胃),同时分别给予生理盐水、碳酸钙阿法D3、健骨灵胶囊、生理盐水灌胃给药。16周后取材,进行逆转录聚合酶链式反应法(RT-PCR)检测小肠组织内VDR mRNA的表达。结果:RT-PCR法检测显示:与空白组比较,模型组VDR mRNA表达水平明显下降(P<0.01);中药干预组及西药对照组用药16周后,与模型组比较,均可明显上调VDRmRNA表达水平(P<0.01);其中中药干预组上调幅度大于西药对照组(P<0.05)。结论:AOP大鼠存在明显的骨代谢紊乱,骨形成减少、骨丢失增加,发生骨质疏松。补肾健脾益气法对AOP大鼠上调小肠组织内VDR mRNA表达,抑制骨丢失,促进骨形成,有效改善实验性AOP大鼠的骨代谢。

Objective ：To observe the influence of reinforcing the kidney and invigorating the spleen to the ex- pression of alcohol - induced Osteoporosis, Vitamin D receptor mRNA and to discuss the pathogenesis of AOP from the gene member level and reinforcing the kidney and invigorating the spleen according to Chinese medi- cine mechanism for prevention and cure. Methods： Divide forty adult male SD rats into four groups by means of random number table： model group, normal control group, western medicine control group and Chinese herbal medicine intervention group with ten rats in each group. Fill the stomach with liquor to make the mold （ normal control group gave normal saline to fill stomach）, separately feeding with normal saline, Calcium Carbonate alpha D3 and JianGuLing capsule. Sixteen weeks later, we measured the weight and checked the expression of Vitamin D receptor mRNA in small intestine tissue, transcriptase polymerase chain reaction. Results： By RT - PCR analysis showed that compared with normal group, model group VDR mRNA expression levels were sig- nificantly decreased（P 〈 O. O1 ） ; compared with model group, Chinese medicine in the intervention group and Western medicine control group medication could significantly increase the level of VDR mRNA expression （P 〈 0.01 ） after 16 weeks ; increasing level of traditional Chinese medicine in the intervention group was greater than that in western medicine control group （ P 〈 0.05 ）. Conclusion ： Rats with AOP suffer bone metabolism disorder, which decreases bone formation and reduces bone loss and finally induces osteoporosis. Reinforcing the kidney and invigorating the spleen on the AOP rats can raise VDR mRNA expression in the small intestine organization,inhibit bone loss and promote bone formation, and effectively improve bone metabolism of the ex- perimental AOP rats.