摘要
目的观察β-细辛醚对抑郁症模型大鼠海马区抗凋亡因子bcl-2表达的影响。方法健康雄性清洁级SD大鼠30只,随机分为正常组、模型组、β-细辛醚组。除正常组外,各组采用孤养加慢性轻度不可预见性刺激方法建立大鼠抑郁症模型,从应激第2天起分别灌胃给药至实验第21天。采用免疫组化法检测大鼠海马区bcl-2的表达。结果与正常组相比,模型组大鼠海马bcl-2表达的平均光密度、阳性细胞数和总面积均明显降低(P<0.01)。与模型组相比,β-细辛醚组能够显著上调大鼠海马区bcl-2表达(P<0.01)。结论β-细辛醚能够上调大鼠海马bcl-2的表达。
Objective Observation β-asarone in depression model rats hippocampal area of anti-apoptotic factor βCL-2 the influence of expression. Methods 30 healthy male Sprague-Dawley rats were randomly divided into normal control group, model control group and β-asarone medicine group. 21d accepted chronic unpredictable mild stress, except the normal control group. In the same time, rats in the model control group and β-asarone medicine group will be given drugs by intragastric separately from the second day to 21th day. After 21 d, we use immunohistochemical method staining in hippocampus tissue of rat brain, to detect the hippocampus BCL-2 expression. Results Compared with nomml control group, model group, the average optical, Positive cell numbers, positive cell aggregate area of bcl-2 activity in hippocampus were significantly decreased （P 〈 0.01 ） ; Compared with model group, the expres- sion of bcl-2 in β-asarone medicine group in hippocampus activity were significantly increased （ P 〈 0. 01 ）. Conclusion β-asa- rone can increase the expression of BCL-2 in rat hippocampa area.
出处
《临床军医杂志》
CAS
2013年第4期329-330,共2页
Clinical Journal of Medical Officers
基金
黑龙江省教育厅课题(编号:12521624)
