一种高效氨氧化细菌扩大培养方法的研究

Study on the Amplification Culture of an High Efficiency Ammonia Oxidizing Bacteria

氨氧化细菌在生物脱氮中扮演重要的角色，它是短程硝化反硝化存在的必要条件，可以提高脱氮效率和节约能耗．具有很好的应用前景。实验针对本公司分离的一株氨氧化细菌CM-014，对其开展扩大培养的研究．提高其氨氮去除效率和生长速度。研究优化氨氧化细菌的培养条件；考察多级种子培养过程中不同移种阶段对扩大培养的影响；研究扩大培养过程中的菌体生长特性和分批补料培养等实验。研究结果表明，CM-014的最佳移种阶段为对数生长后期，优化培养条件为：温度33℃，pH值为7．5-8．0，溶解氧（DO）的质量浓度为1．5．3．0mg／L时，在12d后获得了40L的CM-014富集液，氨氧化活性为7．73mg，（g·h），菌体质量浓度为0．301g／L。

Ammonia Oxidizing Bacteria （AOB） which is necessary for the shortcut nitrification and denitrification plays an important role in biological denitrification. It can improve the nitrogen removal efficiency and reduce the energy consumption, therefore, AOB has a good prospect of application. This study based on a strain of ammonia oxidizing bacteria CM-O14 isolated by our corporation, studied its expansion of culture with the aim to improve its propagation speed and removal efficiency of NH3-N. The culture condition of Strain CM-O14 was optimized; the influence of inoculation phase on propagation during multi-stage seed culture was examined; the growth characteristics and fed-batch culture experiment during propagation process were studied. The resuhs showed that the best stage for transplanting CM-O14 was in the late logarithmic growth phase, conditions of optimization culture were as follows： temperature 33 ℃, pH 7.5 - 8.0, and the mass concentration of dissolved oxygen （DO） 1.5 - 3.0 mg/L. The study received the 40 L CM-O14 enrichment liquid after 12 days, while the activity of the ammonia oxidation was 7.73 mg （NH4＋-N）/[g （MLSS）h], and the cell concentration was 0.301 g （MLSS）/L.