槐米中多糖的提取和含量测定

Extraction and Content Determination of Polysaccharide in Sophora Japhora

目的:以豆科植物槐的花蕾槐米为原料,提取多糖。采用热水提取乙醇沉淀法提取多糖,考查提取纯化条件,确定提取工艺。对提取的多糖进行定性和解析。方法:以苯酚-硫酸法测定多糖含量,制备标准曲线并进行效能指标认证。结果:测得槐米中多糖含量为0.092mg/mg(多糖/原药材)。结论:本研究结果在评价槐米内在质量以及资源开发利用方面具有一定价值。

Objective ：To legume Cassia flower Flos Sophorae buds as raw materials, extraction of polysaecharide. Using hot water extraction and ethanol precipitation method to extract polysaecharide extraction and purification condition,examine, determine the extraction process. On the extraction of polysaccharide and qualitative analysis. Methods： By phenol sulfuric acid method to determine the content of polysaceharide, preparation of standard curve and performance certification. Results： The measured in Flos Sophorae lmmaturus polysaccharide content of 0. 092rag/rag （ palysaeeharide / raw material ） Conclusion ：The results of this study in the evaluation of Flos Sophorae Immaturus internal quality and resource development and utilization value of.