人腺病毒B、D亚种基因组中简单重复序列分析

Analyses of simple sequence repeat in genomes of Human adenoviruses B and D subspecies

本文以人腺病毒B亚种31条基因组序列及D亚种39条基因组序列为研究材料,利用ImperfectMicrosatelliteExtractor和DNAMAN软件对这些基因组序列中简单重复序列(SSR)的分布情况进行了系统性分析和比较。分析结果显示:人腺病毒B、D亚种基因组中简单重复序列的平均相对密度是十分接近的,但在不同类型SSR中分布情况又有所不同。D亚种中二型SSR明显高于B亚种,在两亚种一型SSR中(A)n、(T)n都是比较多的,而在两亚种二型SSR中的(CG/GC)n表现出了较高的偏好性。在同亚种多序列比对分析中,D亚种表现出了更高的稳定性。B、D亚种中SSR的这种特异性分布可能与它们的进化机制和致病性有关。

In our study, the simple sequence repeat in 31 genomes of human adenoviruses B and 39 genomes of hu- man adenoviruses D were analyzed by the software Imperfect Microsatellite Extractor and DNAMAN. The results showed that in human adenoviruses B and D subspecies the average relative density was very similar, but the distri- bution of different repeat motifs was markedly different. The trinucleotide repeats had higher proportion in the D subspecies. In mononucleotide repeats the （A）n and （T）n were predominant and the （CG/GC）n were more in di- nucleotide repeats in Human adenoviruses B and D subspecies. In many sequence alignment analysis of same sub- species, the D subspecies showed higher stability. The specific distribution of SSR may be related to the evolution mechanism and different pathogenicity of Human adenoviruses B and D subspecies.