基于结构的单绕蛋白聚类图构建与分析

Clustering dendrograms construction and analysis of TIM-barrel proteins based on structure information

蛋白质分子进化规律研究是分子进化研究的重点,对揭示生命起源与进化机制有重要意义。本文对已知空间结构及物种信息的单绕蛋白,利用结构比对信息,构建了不同层次单绕样本系统聚类图。分析发现:功能相似蛋白存在明显聚集现象,同一超家族样本基本聚在一个大支中,同一家族样本集中在所属超家族下的小支中,功能约束下单绕样本聚类图与物种进化图有较好对应关系。结果表明:单绕蛋白的结构演化反映了蛋白质功能的约束,特定功能单绕样本的结构差异具有种属特异性,结构演化包含了物种进化信息。

The regularity study of protein molecular evolution is the key to molecular evolution and it has important meaning to reveal life origin and the evolutionary mechanism. In this paper, TIM -barrel proteins with the known spatial structure material and species information were selected as different level datasets to construct clustering dendrograms using their structure alignment information. The analysis of clustering dendrograms shows that proteins with similar function obviously tend to cluster. Members with the same superfamily are almost clustered in a large branch members and the same family with Members are clustered in a small branch which belonged to their super- families. Under the constraints of function, clustering dendrograms of TIM - barrel proteins have a good correspond- ing relation whie with species phylogenies. Our results indicate that the structure evolution of TIM - barrel protein reflects the constraints of protein function, and the structural differences between specific functional TIM - barrel proteins show species specificity. The structure evolution implies the evolution information of species.