不同月龄大鼠勃起功能状况的实验研究

Erectile function of male rats in different age groups: An experimental study

目的:探讨不同月龄组大鼠勃起功能的变化规律,为建立增龄性大鼠ED模型提供动物实验依据。方法:80只雄性Wistar大鼠,分为3、6、12和18月龄组,分别灌胃枸橼酸西地那非进行阴茎勃起功能实验;20只3月龄雌性Wistar大鼠,随机分为4组,建立发情雌鼠模型,观察不同月龄雄鼠的勃起状况与勃起次数。结果:3、6、12和18月龄组Wistar大鼠勃起率及勃起次数分别为85%、75%、40%、30%及(2.27±0.80)、(2.00±0.61)、(1.40±0.51)、(1.29±0.49)次,不同月龄勃起率与勃起次数比较,差异有统计学意义(P<0.05),且随着月龄的增加,各组大鼠勃起功能有降低的趋势。分别将6、12、18月龄组的勃起率及勃起次数与3月龄组大鼠做两两比较,发现6月龄组与12月龄组较3月龄组的差异无统计学意义(P>0.05);18月龄组较3月龄组有明显降低,且差异有显著性(P<0.05)。结论:衰老是大鼠发生ED的主要危险因素,18月龄大鼠已具备建立增龄性大鼠ED模型的条件。

Objective： To explore the relationship between aging and erectile function changes in rats in order to establish a rat model of aging-related erectile dysfunction （ED）. Methods： Eighty male Wistar rats were equally divided into four age groups （3-, 6-, 12- and 18-month） and treated with intragastric administration of sildenafil cihrate （Sn） for penile erection tests. Twenty 3-month- old female Wistar rats were randomized to four groups as oestrous rat models. We recorded the rate and frequency of penile erections of the male rats in different age groups. Results ： The rates of penile erection were 85%, 75% , 40% and 30% and erectile frequencies were 2.27± 0.80, 2.00 ±0.61, 1.40 ±0.51 and 1.29 ± 0.49 in the 3-, 6-, 12- and 18-month rats, respectively, with statisti- cally significant differences among different age groups （P 〈 0.01 ）. And their erectile function exhibited a tendency to decrease with the increase of age. Besides, comparison of the 3-month with the 6-, 12- and 18-month groups showed significantly reduced erectile function in the 18-month rats （ P 〈 0.05 ） but no remarkable difference between the 3-month and the 6- and 12-month groups （ P 〉 0.05 ）. Conclusion ： Aging is one of the main risk factors of rat erectile dysfunction, and 18-month-old male rats are qualified for the establishment of the rat model of aging-related erectile dysfunction.